Objective: To determine if a model of acidosis caused by occlusion of the umbilical cord could be repeated in the same sheep in ethicaly order to reduce the number of animals needed. Method: To obtain fetal acidosis, 1 min of total umbilical cord occlusion (UCO) every 2.5 min was performed on fetal sheep (n=7) until pH reached 7.10. Chronic instrumentation regularly recorded arterial blood gas and hemodynamic parameters. The occlusions were repeated to compare the data between the 2 days for each animal. Results: At the end of the UCO period, fetal acidosis was obtained with a pH 7.14 on day 1 (D1) and 7.06 on day 2 (D2). At baseline, fetuses were more hypotensive at D2 (41 mmHg vs 44 mmHg on D1, p < 0.05). During the UCO period, there was no significant difference in blood gas and hemodynamic data between D1 and D2. Conclusion: To reduce the number of animals needed, performing a second experiment on the same animal appears possible even if results must be interpreted cautiously when severe acidosis is reached.
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Original Research
Fetal acidosis in a sheep model: can we perform a second day of protocol in order to reduce the numbers of animals needed?
C. Pierre1,2,*, L. Ghesquiere1,2, J. De Jonckheere1,3, E. Aubry1,4, D. Sharma1,4, P. Deruelle1,2, L. Storme1,5, V. Houfflin-Debarge1,2, C. Garabedian1,2
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1
University of Lille, EA 4489 – Perinatal Environment and Health, Lille, France
2
CHU Lille, Department of Obstetrics, Lille, France
3
CHU Lille, CIC-IT 1403, Lille, France
4
CHU Lille, Department of Pediatric Surgery, Lille, France
5
CHU Lille, Department of Neonatology, Lille, France
*Correspondence: clementinepierre@hotmail.com (C. PIERRE)
Clin. Exp. Obstet. Gynecol. 2020, 47(1), 79–83;
https://doi.org/10.31083/j.ceog.2020.01.5120
Published: 15 February 2020
Copyright: © 2020 Pierre et al. Published by IMR press.
This is an open access article under the CC BY-NC 4.0 license
https://creativecommons.org/licenses/by-nc/4.0/.
Abstract
Keywords
Fetal sheep
Animal experimentation
Animal ethics
3R
Umbilical cord occlusion
Fetal acidosis
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Figure 1.