Purpose of investigation: Current infertility treatment strategies may result in ovarian hyperstimulation syndrome (OHSS), which can present with hemodynamic instability that involves hemoconcentration, hypoxia, and liver and renal dysfunction that may result from thrombosis. This study’s purpose was to measure the serum biochemical oxidative stress markers in women with severe OHSS. Material and methods: For this prospective controlled study, serum levels of ischemia modified albumin (IMA), total antioxidant capacity (TAC), total oxidative capacity (TOS), oxidative stress capacity (OSI), and serum malondialdehyde (MDA) were measured in women with (n = 25) and without (n = 27) OHSS. Results: In our study, we observed significant differences between the two groups in terms of IMA, TAC, TOS, OSI, and MDA levels. High oxidative stress parameter levels in the OHSS group may indicate that OHSS is an oxidative stress condition. A bivariate correlation analysis revealed a significant correlation between serum TOS level, OSI ratio, and embryo or oocyte quality scores. In addition, there was a negative, non-significant tendency among OHSS patients regarding high IMA, OSI, TOS, and MDA levels and low oocyte and embryo scores. Pregnancy results were not affected in a statistically significantly manner. Conclusion: These results might indicate that oxidative stress status and oxygen radicals may negatively affect ART cycle outcomes.