IMR Press / EJGO / Volume 38 / Issue 6 / DOI: 10.12892/ejgo3718.2017
Open Access Original Research
Stromal cell-derived factor 1 alpha induce epithelial mesenchymal transition of Hela cells
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1 Department of Gynecology, Jing'an District Centre Hospital of Shanghai; Huashan Hospital Fudan University Jing'An Branch, Shanghai, China
2 Department of Obstetric and Gynecology, Taizhou Second Hospital, Taizhou, China
3 Department of Anatomy, Medical College, Soochow University, Suzhou, China
Eur. J. Gynaecol. Oncol. 2017 , 38(6), 933–937; https://doi.org/10.12892/ejgo3718.2017
Published: 10 December 2017
Abstract

Epithelial mesenchymal transition endows cells with migratory and invasive properties. Two groups of Hela cells (invasive group and non-invasive group) were retrieved utilizing Transwell Permeable Supports by Stromal cell-derived factor 1α (SDF-1α). In both the invasive and non-invasive groups, the morphological changes were observed under an inverted microscope, the expressions of mRNA and protein of E-cadherin and Vimentin were analyzed by RT-PCR assay and Western blot, and the levels of c were detected using RT-PCR. The authors found that compared with the non-invasive group, Hela cells in invasive group showed the mesenchymal morphologic changes, decreased expression of E-cadherin mRNA, and protein (p <0.05), increased expression of Vimentin mRNA, and protein (p <0.05), up-regulated expression of Snail, Slug, and Twist mRNA (p <0.05). SDF-1α was able to induce epithelial mesenchymal transition of Hela cells, and obtain the invasive features through up-regulation of Snail, Slug, and Twist.
Keywords
Hela cell
Epithelial mesenchymal transition
E-cadherin
Vimentin
Snail
Twist
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