IMR Press / EJGO / Volume 40 / Issue 4 / DOI: 10.12892/ejgo4760.2019
Open Access Original Research
RNAi silenced NUP88 gene suppresses growth and invasiveness of human breast cancer cell line MCF-7
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1 Department of Clinical Biological Sample Center, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
2 Department of Intensive Medicine, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
3 Department of General Surgery, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
4 Department of Ultrasonography, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
5 Department of Oncology, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China
Eur. J. Gynaecol. Oncol. 2019 , 40(4), 634–639; https://doi.org/10.12892/ejgo4760.2019
Revised: 11 June 2018 | Published: 10 August 2019
Abstract

Objective: To construct and recombine a lentivirus-mediated short hairpin RNA (shRNA) vector targeting NUP88 gene and to observe the effect of silencing NUP88 gene on the biological behavior of MCF-7 cells by RNA interference (RNAi) technology, so as to find a new target for the treatment of breast cancer. Materials and Methods: The recombinant lentiviral vector of NUP88-shRNA was constructed and then transfected into MCF-7 cells. Then, the changes of biological behavior of MCF-7 cells were detected by reverse transcription polymerase chain reaction (RT-PCR), Western-blot, methyl thiazolyl tetrazolium (MTT), flow cytometry, and Transwell assay. Results: The results of RT-PCR and Western-blot showed that the expression of NUP88 mRNA and protein was significantly reduced in NUP88-shRNA group, as compared with that in the blank and negative groups (p < 0.01). The proliferation of MCF-7 cells in the NUP88-shRNA group was prominently reduced after transfected with lentivirus-NUP88-shRNA (p < 0.05). Flow cytometry results showed that the apoptosis rate increased significantly in the NUP88-shRNA group as compared with the other two groups (p < 0.05). After routine culture for 24 hours, the transmembrane cells in the NUP88-shRNA group were notably less than that in the blank or negative control group (p < 0.05). Conclusion: Recombinant NUP88-shRNA lentivirus can successfully inhibit the expression of NUP88 gene in MCF-7 cells and consequently inhibit the proliferation and invasive ability of human breast cancer cells.

Keywords
NUP88
Human breast cancer cell MCF-7
RNAi
Lentiviral vector
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