IMR Press / EJGO / Volume 41 / Issue 4 / DOI: 10.31083/j.ejgo.2020.04.5076
Open Access Original Research
Transmembrane G protein-coupled receptor 30 (GPR30) gene polymorphisms in Korean Women with Ovarian Cancer: An experimental study
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1 Department of Pathology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
2 Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
3 Department of Obstetrics and Gynecology, School of Medicine, Jeju National University, Jeju, Republic of Korea
Eur. J. Gynaecol. Oncol. 2020 , 41(4), 556–562; https://doi.org/10.31083/j.ejgo.2020.04.5076
Submitted: 19 November 2018 | Accepted: 28 January 2019 | Published: 15 August 2020
Copyright: © 2020 Park et al. Published by IMR Press.
This is an open access article under the CC BY 4.0 license https://creativecommons.org/licenses/by/4.0/.
Abstract

Purpose: To compare genetic distribution of fourteen (14) G protein-coupled receptor 30 (GPR30) single-nucleotide polymorphisms (SNPs) in patients with ovarian cancer or benign gynecological disease. Materials and Methods: We examined 117 Korean females with postoperative specimens available for DNA extraction. We used predesigned PCR/Sanger or Sequencing Primer and TaqMan SNP Genotyping Assays for SNP genotyping of the GPR30 gene and performed immunohistochemical staining for assessing GPR30 protein expression. We analyzed linkage disequilibrium (LD) and evaluated differences in genotype and allele frequency between the two groups. Results: We observed no differences in genotype distribution and allele frequency between the two groups. Two LD blocks were determined in both groups. All specimens of both groups revealed intensive staining with a high percentage of positive cells exhibiting GPR30 expression. Conclusions: This study could not demonstrate differences in the genetic distribution of fourteen GPR30 gene polymorphisms in Korean women with ovarian cancer and disease-free controls.

Keywords
Ovarian cancer
G protein-coupled receptor 30
Single-nucleotide polymorphism
Korean
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