IMR Press / EJGO / Volume 41 / Issue 5 / DOI: 10.31083/j.ejgo.2020.05.5314
Open Access Original Research
Downregulation of α-enolase (ENO1) Inhibits Growth, Invasion, and Metastasis of Human Cervical Cancer Cells
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1 Department of Maternal and Child Health Hospital of Zibo City, Zibo, Shandong, 255029, P. R. China.
2 Department of Gynecology and Obstetrics, the Second Affiliated Hospital of Lanzhou University, Lanzhou, Gansu, 730000, P. R. China
3 Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China
4 ICU, Huantai County People's Hospital, Zibo, Shandong, 256400, P. R. China
5 Department of Medical Oncology, Gansu Provincial Tumor Hospital, Lanzhou, Gansu, 730000, P. R. China
6 Institute of Human Anatomy, Histology and Embryology, school of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China
7 Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, United States
8 Department of Obstetrics and Gynecology, Gansu Provincial People's Hospital, Lanzhou, Gansu, 730000, P. R. China
Eur. J. Gynaecol. Oncol. 2020 , 41(5), 762–768; https://doi.org/10.31083/j.ejgo.2020.05.5314
Submitted: 18 June 2019 | Accepted: 15 June 2020 | Published: 15 October 2020
Copyright: © 2020 Zhang et al. Published by IMR Press.
This is an open access article under the CC BY-NC 4.0 license https://creativecommons.org/licenses/by-nc/4.0/.
Abstract

Objectives: The aerobic glycolysis is a characteristic of cancer. α-enolase (ENO1) is an important glycolytic enzyme and is suggested to play a role in the development of tumor. The purpose of this study was to investigate the effects of ENO1 on growth, invasion, and metastasis of human cervical cancer cells. Methods: We knocked down gene coding ENO1 in Hela and SiHa cells with lentivirus, which was confirmed by western-blot. Following downregulation of ENO1, the drug sensitivity, invasion, metastasis, tumourigenic abilities of ENO1-altered cells were investigated. Results: Our studies demonstrated that downregulation of ENO1 enhanced the sensitivity of Hela and SiHa cells to paclitaxel and cisplatin and decreased their tumorigenicity. In addition, the abilities of invasion, metastasis, and tumourigenesis were decreased in ENO1-silenced Hela and SiHa cells. Conclusions: ENO1 is involved in the tumorigenicity, invasion, metastasis, and drug sensitivity of Hela and SiHa cells.

Keywords
α-enolase
Drug sensitivity
Cell tumorigenicity
Invasion and metastasis
Figures
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