IMR Press / FBE / Volume 13 / Issue 2 / DOI: 10.52586/E882
Open Access Article
Brachypodium distachyon ERECTA-like1 protein kinase is a functional guanylyl cyclase
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1 Department of Plant Physiology and Biotechnology, Nicolaus Copernicus University in Torun, Faculty of Biological and Veterinary Sciences, 87-100 Torun, Poland
2 Department of Biology, College of Science and Technology, Wenzhou-Kean University, 325060 Wenzhou, Zhejiang, China
3 Zhejiang Bioinformatics International Science and Technology Cooperation Center, 325060 Wenzhou, Zhejiang, China
Front. Biosci. (Elite Ed) 2021 , 13(2), 249–258; https://doi.org/10.52586/E882
Submitted: 21 September 2021 | Revised: 20 October 2021 | Accepted: 17 November 2021 | Published: 20 December 2021
Copyright: © 2021 The Author(s). Published by BRI.
This is an open access article under the CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/).
Abstract

The plant proteins called ERECTA family play important role in inflorescence architecture, stomatal patterning and phloem-xylem organization. ERECTA proteins belong to the moonlighting proteins family containing the guanylyl cyclase (GC) catalytic center embedded within the intracellular kinase domain. This characteristic architecture of ERECTA proteins prompted us to experimentally confirm of enzymatic activity of one of these, BdERL1 (ERECTA-like1 from Brachypodium distachyon). We have shown that BdERL1 is dual-function protein with both kinase and GC activity. Moreover, our mutagenesis studies also revealed the catalytic roles of key conserved amino acid residues at the GC center and importantly, probing of the kinase and GC with Ca2+ and/or cGMP, shed light on the intramolecular regulations of BdERL1.

Keywords
ERECTA protein kinase
Guanylyl cyclase
Moonlighting proteins
Brachypodium distachyon
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