Academic Editor: Graham Pawelec
Background: One of the major concerns of cancer therapy is the
emergence of multidrug resistance (MDR). The MDR-associated ATP-binding cassette
sub-family B member 1 (ABCB1) transporter is established to mediate resistance
against numerous anticancer drugs. In this study, we demonstrated that the
Ubiquitin-like modifier activating enzyme 1 (UBA1) inhibitor TAK-243 is
transported by the ABCB1. Methods: MTT assay was performed to evaluate
the cytotoxicity of TAK-243. Western blot was carried out to investigate if
TAK-243 affect to ABCB1 protein expression in cancer cells. High Performance
Liquid Chromatography (HPLC) and ATPase assay were carried out to confirm TAK-243
as an ABCB1 substrate. [