Objective: To explore the protective effect of MP on oxidative damage
in vivo and in vitro. Methods: A mouse aging model was induced by
intraperitoneal injection of D-galactose (D-gal), and pathological changes in the
hippocampal ultrastructure were observed by transmission electron microscopy. The
activity of glutathione peroxidase (GSH-Px) and the levels of malondialdehyde
(MDA) in brain tissues were evaluated with GSH-Px and MDA assay kits. An MTT
assay was used to detect the viability of the model SH-SY5Y cells with
HO-induced damage, and a lactate dehydrogenase (LDH) kit was used to evaluate
LDH leakage. Reactive oxygen species (ROS) levels and cell cycle arrest were
analyzed by flow cytometry, and cleaved caspase 3 and P53 protein expression was
determined by western blot analysis. Results: Demonstrated that MP
increased GSH-Px activity, reduced MDA levels, and attenuated the cell damage
induced by HO. Furthermore, MP protected neuronal cells from oxidative stress
through a mechanism including a decrease in LDH leakage and reversal of
HO-induced cell morphological damage. MP treatment alleviated the HO-induced
increases in ROS levels, inhibited apoptosis, relieved cell cycle arrest, and
downregulated cleaved caspase 3 and P53 protein expression. Conclusions:
MP is a novel antioxidant with neuroprotective effects.