IMR Press / JIN / Volume 20 / Issue 4 / DOI: 10.31083/j.jin2004089
Open Access Original Research
Variable neuroprotective role of Filipendula ulmaria extract in rat hippocampus
Show Less
1 Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
2 Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
3 Department of Science, Institute for Information Technologies Kragujevac, University of Kragujevac, 34000 Kragujevac, Serbia
4 Department of Chemistry, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia
5 Department of Pathology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
6 Department for Biology and Ecology, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia
7 Department of Genetics, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
8 BioIRC, Bioengineering R&D Center, 34000 Kragujevac, Serbia
9 Faculty of Engineering, University of Kragujevac, 34000 Kragujevac, Serbia
10 Department of Human Pathology, First Moscow State Medical University IM Sechenov, 119992 Moscow, Russia
11 Department of Histology and Embryology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia

These authors contributed equally.

J. Integr. Neurosci. 2021 , 20(4), 871–883; https://doi.org/10.31083/j.jin2004089
Submitted: 2 July 2021 | Revised: 19 August 2021 | Accepted: 30 August 2021 | Published: 30 December 2021
Copyright: © 2021 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/).
Abstract

We evaluated the influence of an antioxidant-rich extract of Filipendula ulmaria L. on anxiety levels induced by nano-sized particles of different calcium phosphates. Rats in experimental groups were administered with either nano-sized hydroxyapatite, tricalcium phosphate, or amorphous calcium phosphate in the presence of Filipendula ulmaria extract. Appropriate behavioral tests were performed to assess anxiety levels, while oxidative status and apoptosis parameters were determined in the hippocampus samples. The applied calcium phosphates increased oxidative stress markers in hippocampal tissue, accompanied by an enhanced pro-apoptotic mechanism. Moreover, the hippocampal immunoreactivity for brain-derived neurotrophic factor and GABAergic-A receptors was significantly lower following calcium phosphate nanoparticles intake. The observed functional and morphological alterations in the rat hippocampus occurred simultaneously with the anxiogenic response estimated in behavioral testing. The neuroprotective effect of Filipendula ulmaria was markedly manifested by the attenuation of oxidative damage induced by amorphous calcium phosphate and enhanced anti-apoptotic action in the rat hippocampus. The increased hippocampal immunoreactivity for brain-derived neurotrophic factor, GABAergic-A receptors and significant anxiolytic-like effects of Filipendula ulmaria may suggest a beneficial role of antioxidant supplementation in preventing anxiogenic response to nano-sized calcium phosphates.

Keywords
Calcium phosphates nanoparticles
Filipendula ulmaria
Behavior
Anxiety
Apoptosis
Oxidative stress
Figures
Fig. 1.
Share
Back to top