This systematic review aims to compare and evaluate the outcome of using either testicular sperm (Testi-ICSI) or ejaculated sperm (Ejac-ICSI) in intracytoplasmic sperm injections in patients with recurrent ICSI failure. The Cochrane Library, MEDLINE, EMBASE, and PubMed were used to search for relevant papers up till October 2020. Four cohort studies and two case series studies were included. Four studies investigated males with high sperm DNA fragmentation (SDF) and were classified as “high SDF”, which included 247 couples and 2712 injected oocytes. The other three studies provided paired data to an unselected population of infertile men with either untested SDF or when anomalous SDF was not used as the basis for deciding to use Testi-ICSI, and were classified as “noclassify” in this study. This subgroup consisted of a total of 290 couples and 1061 injected oocytes. There was a higher level of clinical pregnancy rates (CPRs) in the “high SDF” subgroup when Testi-ICSI was used as compared to Ejac-ICSI, at 43.4% and 20.8% respectively, with a pooled odds ratio (OR) of 2.87 (95% confidence interval (CI) 1.44–5.71; P = 0.003). Furthermore, in the “high SDF” subgroup, Testi-ICSI use was associated with better take home baby rates (38%) as compared to Ejac-ICSI (16%), with a pooled OR of 3.24 (95% CI 1.20–8.76; P = 0.02). In the “noclassify” group, there was no statistically significant difference in the CPRs and take home baby rates of Testi-ICSI and Ejac-ICSI, although there was a trend of better CPRs and take home baby rates with Testi-ICSI use. Utilization of Testi-ICSI in recurrent ICSI failure couples, where males were confirmed to have high SDF in their ejaculated sperm, were correlated with greater CPRs and take home baby rates. However, Testi-ICSI may not result in better ICSI outcomes among men with untested SDF or when anomalous SDF was not the main factor influencing the decision to utilize Testi-ICSI.
About 40% of couples seeking assisted reproductive technology (ART) tend to
have repeated miscarriages and implantation failures . Sperm DNA fragmentation
(SDF) may contributes to fertilization capacity and embryo development .
However, as there is a lack of evidence on the relationship between SDF and
reproductive outcomes, the American Society of Reproductive Medicine (ASRM) does
not encourage the routine use of SDF assays in patients who are considering ART
. In contrast, recent evidence supports the role of SDF in ART: in infertile
males with SDF
Testicular sperm extraction (TESE), microscopic TESE (microTESE), or testicular sperm aspiration (TESA) are methods commonly utilized to harvest testicular sperm. When the non-obstructive azoospermia leading to infertility occurs, and sperm donor assistance is refused, testicular retrieved sperm are conventionally used . During the course of an ART cycle, the source of sperms could possibly influence SDF levels . In addition, previous systematic reviews have shown that testicular sperm had lower SDF levels as compared to ejaculated sperm, and the using of testicular sperm for ICSI was more likely to lead to better results [7,8]. Hence, once the presence of high SDF is confirmed in ejaculated sperm, testicular sperm DNA is considered to be more complete than ejaculated sperm.
However, testicular sperm extraction is invasive, and complications such as bleeding, infection, and irreversible testicular tissue damage may potentially occur during the operation . Hence a careful evaluation should be made as to whether testicular sperm should be used. This study thus aims to collect and summarize the evidence on whether couples with recurrent ICSI failure will benefit more from Testi-ICSI as compared to Ejac-ICSI. Clinical Pregnancy Rates (CPRs) and take home baby rate were defined as the primary outcomes, and the fertilization rate was used as the secondary outcome.
As the present study involved no human intervention, the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) statement was adhered to in reporting the results. The study was also registered with PROSPERO (registry number: CRD42021225964).
The Cochrane Library, Google Scholar, and Pubmed, were used to identify all relevant studies until October 2020. The search combined terms related to “testicular sperm”,“ejaculate”,“ICSI failure OR ART failures”, and “intracytoplasmic sperm injection”, combined with the filter “human” in any language and article types. Finally, references of included articles were also manually searched to identify relevant studies.
Studies that evaluated the effect of Testi-ICSI and Ejac-ICSI among couples with history of ICSI failure were included, with or without SDF test in the ejaculate sperm. Exclusion criteria included the following: (1) Diagnosis of azoospermia or cryptozoospermia; (2) Review articles; (3) Use of other therapeutic strategies (medication, varicocelectomy) before baseline; (4) Missing outcome data. Abstracts were independently evaluated by two authors (YHL and XWY), and full manuscripts were retrieved if they met the selection criteria. If any discrepancies occurred, a third author (WQ) was involved.
Data was extracted independently by two authors (XYZ and YHL). For each study, specific information were identified and extracted as follows: (1) Population characteristics (history of ICSI failure); (2) Participants’ semen analysis profile; (3) Study design; (4) Sperm extraction method; (5) Age of participants; (6) Outcome data (fertilization rates, CPRs, and take home baby rates. Nonrandomized studies were also assessed for the risk of bias  as shown in Supplemental Table 1.
The leave-one-out approach was used for sensitivity analyses. When individual studies were excluded in turn, there was lead no significant change in summarized conclusion. This signifies that the results were reliable. Funnel plot asymmetry tests were not conducted for the assessment of publication bias as the number of included trials were too small.
Review Manager 5.3 (The Nordic Cochrane Centre, The Cochrane Collaboration,
Copenhagen, Denmark) was utilized to conduct statistical analyses. For
dichotomous variables, odds ratio (OR) and the 95% confidence interval (CI) were
calculated whereas for continuous variables, the standardized mean difference
(SMD) and 95% CI were used. Q-test or I
After the initial identification of 198 studies, 4 cohort studies [12-15] and 2 case series studies [16,17] published between 2005 and 2020 were included in the final analysis (Fig. 1). The pertinent characteristics of each study are shown in Table 1 (Ref. [12-17]). The two subgroups of “high SDF” and “noclassify” are presented in the forest plots as previously mentioned.
Trial identification and selection process.
|Author (year)||Patients included||Male age (Year)||Female age (Year)||Sperm extraction||Design||Outcome measures||Previous ICSI failures|
|Greco 2005 ||High SDF: (TUNEL)||28–55||24–35||TESE||Case-control study||A, B|
|SDF rates in neatsemen
|Pabuccu 2017 ||High SDF: (TUNEL)||40.1
||TESA||Case-control study||A, B, C|
|Normozoospermic with SDF
|High SDF: (SCD)||47.5
||TESA||Case-control study||A, B, C||At least 1 ICSI failure with ejaculated sperm|
|Arafa 2017 ||Normozoospermic with SDF
|Gilman 2018 ||Noclassify; (mean)||40.6
||Micro-TESE||Retrospectivecohort||A, B, C||1 ICSI failure with ejaculated sperm|
|Sperm conc (M/mL): 29.6|
|Sperm motility (%): 25.5|
|Sperm morphology: 3|
|Herrero 2019 ||Noclassify; (mean)||40.1
||TESE||Case-control study||A, B, C|
|Sperm conc (M/mL): 37.6|
|Sperm motility (%): 23.5|
|Sperm morphology: 4.2|
|Alharbi 2020 ||High SDF:||37.0
||TESA||Retrospectivecohort||A, B, C||At least 1 ICSI failure with ejaculated sperm|
|Normozoospermic with SDF
|E-ICSI, ICSI using ejaculated sperm; ICSI, intracytoplasmic sperm injection; SCD, sperm chromatin dispersion; TESA, testicular sperm aspiration; TESE, testicular sperm extraction; T-ICSI, ICSI using testicular sperm; TUNEL, transferase-mediated dUTP nick-end labeling. A: Fertilization rates; B: Clinical pregnancy rate; C: Take home baby rate.|
Fertilization rates were provided in 6 studies, with a total of 3773 injected
oocytes. In both subgroups of “high SDF” and “noclassify” there was no
heterogeneity. In the “high SDF” subgroup, fertilization rate was 59.1% for
Testi-ICSI and 58.5% for Ejac-ICSI, with a pooled OR of 1.04 (95% CI 0.89–1.21;
Forest plot demonstrating relative risk for fertilization rates with intracytoplasmic sperm injection (ICSI) when using testicular or ejaculated sperm.
CPRs were provided in 6 studies, over 465 cycles. For the “noclassify”
subgroup, CPRs were 35.3% and 28.1% for Testi-ICSI and Ejac-ICSI respectively,
with a pooled OR of 1.39 (95% CI 0.81–2.37; I
Forest plot demonstrating relative risk for Clinical Pregnancy Rates with Testi-ICSI and Ejac-ICSI.
Five studies reported take home baby rates, over a total of 484 couples. Take
home baby rates were much higher among Testi-ICSI couples as compared to
Ejac-ICSI couples in the “high SDF” subgroup, at 38% and 16% respectively,
with an a pooled OR of 3.24 (95% CI 1.20–8.76; I
Forest plot demonstrating relative risk for take home baby rates with Testi-ICSI and Ejac-ICSI.
Many meta-analyses and systematic reviews have compared between the use of testicular sperm (Testi-ICSI) and ejaculated sperm (Ejac-ICSI) in intracytoplasmic sperm injection in patients without azoospermia , with high SDF [7,8] and cryptozoospermia [19-21]. None of these meta analyses focused on the benefits of Testi-ICSI in couples who experienced previous ICSI failures, except for Esteves et al.  who evaluated the benefits of Testi-ICSI among males with high SDF. In addition, only two articles provided data on a subgroup of participants with “repeat ICSI failure”, which was used by Esteves et al. for further analyses. Since the publication of these meta-analyses, additional studies have been published [12-14,16], which provide new information with regards to the use of Testi-ICSI and Ejac-ICSI.
Studies have shown ART outcomes tends to be negatively affected by impaired sperm, although some couples can have successful pregnancies even when using abnormal sperm [22,23]. For men with higher SDF levels in their ejaculated sperm, the use of Testi-ICSI was associated with a higher rates of implantation and pregnancy than Ejac-ICSI, as testicular sperm tends to have a lower SDF as compared to ejaculated sperm [7,24]. This may possibly be due to the difference in SDF between testicular and ejaculated sperm, as infertile men were more likely to have abnormal SDF levels, even within normal semen parameters [25,26].
As opposed to ejaculated sperm, this possibly hints at the ability of testicular sperm, in achieving more favorable reproductive outcomes in couples who have experienced previous ICSI failures. To our knowledge, the present study is the first to focus mainly on the use of Testi-ICSI in couples with recurrent ICSI failure. Our results show that if ejaculated sperm was found to have high levels of SDF, Testi-ICSI was associated with higher CPRs and take home baby rates. However, in an unselected population of infertile men, with untested SDF, Testi-ICSI may not result in better ICSI outcomes. This is consistent with a previous meta-analysis which examined men with cryptozoospermia, and found that Testi-ICSI did not result in a better outcome than Ejac-ICSI, where there was insufficient sperm to detect SDF levels .
The failure of fail one or several IVF cycles does not predict failure in subsequent cycles . Some researchers insist that the conclusion of Testi-ICSI leads to better reproductive outcomes in recurrent ICSI failure unreliable, as the Testi-ICSI cycle was compared with those of the first failed Ejac-ICSI cycle for the same patient in the case series studies. Furthermore, couples who achieved pregnancy with Ejac-ICSI were excluded . The use of case-control studies have solved this problem, because Testi-ICSI cycles are not compared with previous Ejac-ICSI failure cycle within the same patient, but with Ejac-ICSI cycles in other couples with comparable conditions.
Some researchers disagree with the use of Testi-ICSI in nonazoospermic men due to the possible surgical complications . In addition, while Moskovtsev et al.  showed that aneuploidy rates were 2 to 3 times higher in testicular sperm than ejaculated sperm, others found that testicular and ejaculated sperm had similar aneuploidy rates . Currently available aneuploidy studies tend to be conducted with a smaller sample size, thus the question of whether testicular sperm truly has a higher rate of aneuploidy remains inconclusive.
The most common assays, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), sperm chromatin dispersion (SCD), Comet-single cell gel electrophoresis, and sperm chromatin structure assay (SCSA) were mainly used to detect SDF clinically . Most of the studies included in our meta-analysis used the TUNEL method, with the threshold set at 15%  and 30% , whereas another article used the SCD method, and set the threshold as 30% . In Esteves et al.’s  study, 29% was deemed to be the threshold of SDF, where Testi-ICSI becomes more beneficial. Due to the lack of standardized programs and the different measurement methods used, the sensitivity, specificity, and cut-off rate of each method varies. In addition, the type of sperm DNA detection and threshold that should be used remains controversial [30,31]. This problem also exists in the present study, which may weaken the strength of the conclusions drawn. Therefore, the use of reliable SDF measurements and effective thresholds are essential, and the limitations of SDF testing and the possible benefits to clinical results should be taken into account when considering the use of Testi-ICSI.
Our study aimed to evaluate the influence of the source of sperm (testicular or
ejaculated) used for ICSI on ICSI results for couples with history of ICSI
failure. Thus, factors concerning the female, such as her age, AMH, BMI should be
comparable between the different experimental groups to prevent bias. In our
study, two of included studies investigated the outcome of subsequent Testi-ICSI
cycles for couples who failed their initial Ejac-ICSI [16,17]. For the rest of
the included studies, only one study clearly stated that couples with an older
The present study has limitations. Firstly, a number of the included studies had small sample sizes and all studies were retrospective in nature. Secondly, certain factors that could have influenced ICSI outcomes were not reported in all studies, such as the lifestyle patterns of participants, presence of varicoceles, and medication use, along with other relevant male factors. Furthermore, different detection methods and cutoff values were used to determine the percentage of sperm with fragmented DNA. In addition, the definition of recurrent ICSI failure differs among the included studies; Finally, a potential confounder was identified in Herrero et al.’s  study, where the Testi-ICSI group had a significantly higher SDF than the Ejac-ICSI group’s SDF. Although sensitivity analyses showed no change in results, the presence of such confounders may result in bias.
In this article, we evaluated whether the outcomes of Testi-ICSI were superior to that of Ejac-ICSI in recurrent ICSI failure couples. The analyses showed that the use of Testi-ICSI in recurrent ICSI failure couples, where the ejaculated sperm of males had high levels of SDF, was related to better CPRs and LBRs. However, when Testi-ICSI was used in an unselected population of infertile men with either untested SDF or when their decision to use Testi-ICSI was not based on anomalous levels of SDF, treatment with Testi-ICSI may not result in better outcomes. When genomics, epigenetics, proteomics, and metabolomics are better understood in the future, it may then be possible to answer the question of whether sperm in the testis is truly better than sperm from the ejaculate.
Currently, there is limited research on Testi-ICSI use in couples with recurrent ICSI failures. The present study suggests that Testi-ICSI use was correlated with higher CPRs and LBRs only in males with confirmed high SDF levels in their ejaculated sperm. However, when Testi-ICSI was used in an unselected population of infertile men with either untested SDF or when their decision to use Testi-ICSI was not based on anomalous levels of SDF, treatment with Testi-ICSI may not result in better outcomes.
ART, assisted reproductive treatment; CIs, confidence interval; CPRs, Clinical Pregnancy Rates; ICSI, intracytoplasmic single sperm injection; IVF, in vitro fertilization; ORs, Odds ratios; SDF, Sperm DNA fragmentation; SMD: standardized mean difference; Testi (Ejac)-ICSI, testicular sperm (ejaculated sperm) intracytoplasmic single sperm injection.
QW and XWY designed and instructed the writing of this article. YHL and XYZ researched and assessed the literature. QW, XYZ and XWY extracted the data from each article and drew the Tables and Figures of the review. All authors read and approved the final manuscript.
Research involving human participants or animals were not conducted by any of the authors. The study was also registered with PROSPERO (registry number: CRD42021225964).
Thanks to all the peer reviewers for their opinions and suggestions.
Study on the effect of oxLDL on progesterone production by HIF pathway on infertility in obese women-Jilin Provincial Finance Department (JLSCZD2019-040).
The authors declare no conflict of interest.
Supplementary material associated with this article can be found, in the online version, at https://oss.jomh.org/jomh/article/1402519669519073280/attachment/Supplemental%20Table2ROBINS-I.docx.