IMR Press / RCM / Volume 22 / Issue 3 / DOI: 10.31083/j.rcm2203103
Open Access Original Research
Abnormal expression of TGFBR2, EGF, LRP10, and IQGAP1 is involved in the pathogenesis of coronary artery disease
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1 Department of Respiratory and Critical Care Medicine, the 2nd Hospital of Jilin University, 130041 Changchun, Jilin, China
2 Department of Prescriptions, Pathology and Pathophysiology, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, China
3 Department of Nursing, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, China
These authors contributed equally.
Rev. Cardiovasc. Med. 2021 , 22(3), 947–958; https://doi.org/10.31083/j.rcm2203103
Submitted: 22 February 2021 | Revised: 14 April 2021 | Accepted: 15 July 2021 | Published: 24 September 2021
Abstract

Coronary artery disease (CAD) is the most common cardiovascular disease worldwide. In this study, we investigated the pathogenesis of CAD. We downloaded the GSE98583 dataset, including 12 CAD samples and 6 normal samples, from the Gene Expression Omnibus (GEO) database and screened differentially expressed genes (DEGs) in CAD versus normal samples. Next, we performed functional enrichment analysis, protein-protein interaction (PPI) network, and functional module analyses to explore potential functions and regulatory functions of identified DEGs. Next, transcription factors (TFs) and microRNAs (miRNAs) targeting DEGs were predicted. In total, 456 DEGs were identified in CAD and normal samples, including 175 upregulated and 281 downregulated genes. These genes were enriched in the intestinal immune network for immunoglobulin A production and the mitogen-activated protein kinase signaling pathway (e.g., TGFBR2 and EGF). The PPI network contained 212 genes, and HIST1H2BJ, HIST1H2AC, EGF, and EP300 were hub genes with degrees higher than 10. Four significant modules were identified from the PPI network, with genes in the modules mainly enriched in the inflammatory response, protein ubiquitination involved in ubiquitin-dependent protein catabolic processes, protein transport, and mitochondrial translational elongation, respectively. Two TFs (E2F1 and FOXK1) and five miRNAs (miR-122A, miR-516-5P, miR-507, miR-342, and miR-520F) were predicted to target 112 DEGs. miR-122A reportedly targets both LRP10 and IQGAP1 in the TF-miRNA target regulatory network. The abnormal expression of TGFBR2, EGF, LRP10, and IQGAP1 may be implicated in CAD pathogenesis. Our study provides targets and potential regulators for investigating CAD pathogenesis.

Keywords
Coronary artery disease
Transcription factor
microRNAs
MAPK signaling pathway
Pathogenesis
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