Objective: To investigate the role and possible molecular mechanism of the transcription factor Krüppel-like factor 4 (KLF4) in the biological phenotype of endometrial cancer cells following induction by oleic acid (OA). Materials and Methods: Ishikawa endometrial cancer cells were grown in vitro with different concentrations of oleic acid. Cell proliferation was measured using the Counting Kit-8 (CCK-8) assay. Cell migration and invasion were evaluated by transwell chamber assay and scratch assay, respectively. The mRNA and protein expression levels of KLF4, ERα, Bcl2, MMP1, MMP9, were evaluated by qRT-PCR and Western blot, respectively. Results: The expression levels of KLF4, ERα, Bcl2, MMP1 and MMP9 increased significantly (p < 0.05) upon stimulation of the endometrial cancer cells with oleic acid (200 µM), together with the migration and invasion abilities of the cells (p < 0.05). Up-regulation of KLF4 caused the expression of ERα, Bcl2 and MMP9 to increase significantly (p < 0.05), whereas down-regulation of KLF4 caused the expression of ERα, Bcl2, MMP1 and MMP9 to significantly decrease (p < 0.05). The increased expression levels of ERα, Bcl2, MMP1 and MMP9 upon 200 µM oleic acid stimulation were significantly blocked (p < 0.05) by down-regulation of KLF4. Conclusion: oleic acid promotes the expression of ERα, Bcl2, MMP1 and MMP9 via up-regulation of KLF4, thus resulting in increased migration and invasion abilities of endometrial cancer cells.
Cite this article
Oleic acid promotes the development of endometrial cancer by up-regulating KLF4 expression
1 Shihezi University School of Medicine, Shihezi, Xinjiang 832000, China
Eur. J. Gynaecol. Oncol. 2020 , 41(3), 432–438; https://doi.org/10.31083/j.ejgo.2020.03.5241
Submitted: 11 April 2019 | Accepted: 15 July 2019 | Published: 15 June 2020