Purpose: To evaluate intraovarian histologic changes caused by polypropylene and silk sutures that commonly are used in ovarian suspension. Materials and Methods: Twenty-four female rats were randomly allocated to three study groups: a sham group receiving no ovarian suspension; the other two groups that had right ovarian suspension with polypropylene and silk sutures. At 90 days after surgery, the histologic changes and ovarian weight reduction in the suspended ovaries and severity of pelvic adhesions were evaluated. Results: There were no differences between study groups in focal inflammation, cystic structures, or vascularity. Adhesion severity and ovarian weight reduction in suspended ovaries and cysts around the suspended ovary were significantly greater in the silk than sham group. The frequency of hematoma within the suspended ovary was significantly greater in the polypropylene than sham group. Conclusions: Polypropylene suture caused less adhesion severity or ovarian weight reduction than silk suture. This suggests that polypropylene suture may be the better suture for ovarian suspension procedures.