Background: We investigated the susceptibility patterns and genotype of extended-spectrum-beta-lactamase (ESBL)-producing Klebsiella pneumoniae isolates. Methods: A prospective survey of K. pneumoniae-infected patients was conducted in obstetrics & gynaecology settings in three associated hospitals of a university in China from 2017 to 2019. All isolates were identified as K. pneumoniae by the conventional standard procedures and confirmed by use of the VITEK GNI system. Susceptibility to 16 antimicrobial agents of ESBLs was determined by way of a screening procedure using discs. PCR amplification of bla genes, and sequencing of PCR products were performed to determine their molecular types. Results: A total of 518 of K. pneumoniae were isolated from sputum specimens in three affiliated hospitals. Out of these, 158 strains were ESBL producers and 64 strains were non-ESBL producers from the first hospital; 114 strains were positive for producing ESBLs and 60 strains were negative for ESBLs in the second hospital, and 86 strains were ESBL producer and 36 strains were negative from the third hospital. bla gene contents showed that 88 strains (24.6%) carried the bla{}_{T\mathit{}E\mathit{}M\mathrm{-}\mathrm{2}} gene, and 74 strains (20.1%) contained the bla{}_{S\mathit{}H\mathit{}V\mathrm{-}\mathrm{3}} gene, 44 strains (12.3%) had the bla{}_{C\mathit{}T\mathit{}X\mathrm{-}M\mathrm{-}\mathrm{2}} gene, 42 strains (11.7%) had the bla{}_{C\mathit{}T\mathit{}X\mathrm{-}M\mathrm{-}\mathrm{4}} gene, 38 strains (10.6%) harboured bla{}_{C\mathit{}T\mathit{}X\mathrm{-}M\mathrm{-}\mathrm{5}} and bla{}_{C\mathit{}T\mathit{}X\mathrm{-}M\mathrm{-}\mathrm{8}} genes, and 29 (9.5%) strains contained bla{}_{C\mathit{}T\mathit{}X\mathrm{-}M\mathrm{-}\mathrm{15}} gene. Also, 20 (5.6%) strains contained bla{}_{O\mathit{}X\mathit{}A\mathrm{-}\mathrm{1}}. The co-existence of bla{}_{C\mathit{}T\mathit{}X\mathrm{-}M\mathrm{-}\mathrm{15}} and bla{}_{S\mathit{}H\mathit{}V\mathrm{-}\mathrm{3}} was identified in 20 strains (5.6%), 12 strains (3.3%) co-existed with bla{}_{S\mathit{}H\mathit{}V\mathrm{-}\mathrm{3}} and bla{}_{T\mathit{}E\mathit{}M\mathrm{-}\mathrm{2}}, 14 isolates (3.9%) harboured bla{}_{T\mathit{}E\mathit{}M\mathrm{-}\mathrm{2}} and bla{}_{C\mathit{}T\mathit{}X\mathrm{-}M\mathrm{-}\mathrm{15}}, eight isolates (2.2%) possessed bla{}_{S\mathit{}H\mathit{}V\mathrm{-}\mathrm{3}}, bla{}_{T\mathit{}E\mathit{}M\mathrm{-}\mathrm{2}}, and bla{}_{C\mathit{}T\mathit{}X\mathrm{-}M\mathrm{-}\mathrm{15}}. Discussion: The high prevalence of ESBLs in K. pneumoniae isolates with diverse enzyme gene types indicated the need for screening the changes in ESBL-producing isolates in this region.