- Academic Editor
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†These authors contributed equally.
Background: This project aimed to investigate the role of two
arachidonic acid metabolites (14,15-epoxyeicosatrienoic acid (14,15-EET) and
15-hydroxyeicosateraenoic acid (15-HETE)) as a precursor of hypertensive
disorders in pregnancy by comparing their serum levels between third-trimester
hypertensive and normal pregnant women. The relationship between their
differential levels and pregnancy outcomes was investigated to clarify the role
of arachidonic acid metabolites in the occurrence and development of hypertensive
disorders during pregnancy. Methods: This is a prospective cohort study
and a total of 88 patients were included in the study. 17 of them were recognized
as the gestational hypertension and 25 of them were considered to be the
preeclampsia. 24 women were diagnosed with severe preeclampsia. The control group
consisted of 22 healthy patients pregnancy course, with no disease at the present
and in the history. For this purpose, the serum levels of 14,15-EET and 15-HETE
of gestational hypertension, preeclampsia (PE) and normal pregnant women group
were detected by enzyme-linked immunosorbent assay (ELISA) during the third
trimester of pregnancy. It was followed by detecting placental cytochrome P450
2J2 (CYP2J2) and 15-lipoxygenase-2 (15-LOX-2) expression and localization using
immunohistochemistry, their corresponding proteins employing western blotting.
All outcomes of maternal pregnancy were then statistically analyzed.
Results: The analysis indicated that the levels of 14,15-EET, and
15-HETE were significantly higher (p