- Academic Editors
Background: Current evidence suggests that Gamma-aminobutyric acid
(GABA) receptors are associated with the occurrence and progression of
cerebrovascular diseases. The present study investigated the association between
single nucleotide polymorphisms (SNPs) in the Gamma-aminobutyric acid type A
receptor gamma2 subunit (GABRG2) gene and ischemic stroke (IS).
Methods: A total of 120 healthy volunteers and 187 patients with IS were
recruited. Patients underwent complete neurological assessment and classification
with the National Institute of Health Stroke Scale (NIHSS) and the Trial of ORG
10172 in Acute Stroke Treatment (TOAST). Polymerase chain reaction-restriction
fragment length polymorphism (PCR-RFLP) was used to analyze SNP sites in 4
different regions (rs211037, rs418210, rs211035, and
rs424740) of the GABRG2 gene. SHEsis online platform was used
to assess SNP allele and genotype frequencies. Multivariate logistic regression
analysis was performed to identify the risk factors for IS. Results:
Univariate analysis showed that the T allele and
TT genotype distribution for rs211037 were significantly more
frequent in the IS group compared to controls (p
Stroke is one of the common cerebrovascular diseases and can be divided into hemorrhagic stroke and ischemic stroke (IS) [1]. The morbidity and mortality from stroke have decreased with the improvement of medical care, but the absolute number of patients with this disease is still increasing yearly due to aging of the global population [2, 3]. Nearly 80% of stroke patients are diagnosed with IS [1]. Although the pathogenesis of IS has not been fully elucidated, genetic susceptibility genes have been suggested as key factors in its etiology [4]. Many susceptibility factors for IS have been reported, but these only partially explain the genetic risk for IS. The remainder can be attributed to other covariates, such as blood lipids, diabetes and blood pressure [5, 6]. Therefore, in-depth exploration of the genetic background of IS and the finding of new susceptibility factors has high clinical value for the early diagnosis, treatment, and prognosis of IS patients.
Gamma-aminobutyric acid (GABA) acts as an inhibitory neurotransmitter in the
central nervous system. The GABA type-A receptor
Single nucleotide polymorphisms (SNPs) are third-generation genetic diagnostic biomarkers that have been used widely in many fields of biology and medical research [14]. SNPs in GABAR2 have been associated with nervous system diseases, including epilepsy [15, 16, 17]. The GABRG2 C588T polymorphism is a potential predictor of generalized epilepsy risk [15]. The rs211037 SNP in the GABRG2 gene has also been associated with the risk of idiopathic generalized epilepsy [16]. The association of GABRG2 SNPs with the risk of IS in humans has not been investigated, however. Moreover, associations between GABRG2 SNPs and autoimmune associated diseases, including IS, have not been proven conclusively. In the present study we investigated possible associations between 4 SNPs (rs211037, rs418210, rs211035, and rs424740) in GABRG2 and the susceptibility to IS in a Chinese population.
This case-control study included 187 patients with atherosclerotic acute IS. The inclusion criteria were: (1) IS confirmed by cerebral magnetic resonance imaging (MRI); (2) cases were classified as large-artery atherosclerosis (LAA), cardiogenic embolism (CE) or small artery occlusion (SAO) based on Trial of ORG 10172 in Acute Stroke Treatment (TOAST). Exclusion criteria were: (1) family history of stroke or previous stroke; (2) other type of stroke; (3) cerebral hemorrhage, severe heart and liver disease, blood or autoimmune diseases, chronic inflammatory diseases; (4) inability to perform MRI imaging; (5) patients related to each other.
During the same period, 120 healthy volunteers were recruited to our hospital for physical examination and served as the control group. None of the control volunteers had a computer tomography (CT), MRI or family history of stroke. Healthy volunteers who participated in the study had no tumors, atherosclerotic disease, immune-related diseases, infections, severe heart disease, severe liver and kidney dysfunction, or endocrine disorders. The study protocol was approved by the Medical Ethics Committee of Red Cross Hospital (Approval NO: 2023030). Patients and/or their families gave informed consent for samples to be collected and analyzed.
Data were collected for a variety of clinical characteristics including age, gender, smoking, drinking history, diabetes and hypertension. Fasting blood samples were tested for glucose, triglycerides (TG), plasma total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C).
The National Institute of Health Stroke Scale (NIHSS) evaluates 11 factors, including consciousness level, muscle strength, aphasia, ataxia, and sensation. The higher the score, the worse the neurological function. Scores represent mild (1–4), moderate (5–15), moderate to severe (16–20), or severe (21–42) neurological disability [18].
The following SNPs in the GABRG2 gene (locus gene frequency
Fasting venous blood (4 mL) was collected into an EDTA tube and centrifuged at 4
°C 1500
SNP | Primer sequence | |
rs211037 | sense | 5 |
anti-sense | 5 | |
rs418210 | sense | 5 |
anti-sense | 5 | |
rs211035 | sense | 5 |
anti-sense | 5 | |
rs424740 | sense | 5 |
anti-sense | 5 |
SNP, Single nucleotide polymorphism.
Data visualization and analysis were performed using GraphPad Prism 6.0
(GraphPad Software, Inc., San Diego, CA, USA) and SPSS 21.0 (IBM Corp., Armonk,
NY, USA). The normality of measurement data was analyzed by F test, and Student
t test was used to evaluate differences between two groups. Comparison
of numerical data between two groups was conducted using the Chi-square test.
Odds ratio (OR) and 95% confidence intervals (CI) were calculated using logistic
regression analysis. Genotyping results for the 4 SNPs in GABRG2
(rs211037, rs418210, rs211035, and rs424740)
were analyzed with the SHEsis online platform
(http://analysis.bio-x.cn/myAnalysis.php). Hardy-Weinberg equilibrium (HWE),
linkage disequilibrium and haplotype analysis were also carried out. Indicators
of significant differences between the IS and control groups were taken as
covariates, with the incidence of IS taken as the dependent variable. The
relationship between the rs211037 genotype and IS was analyzed using
multivariate logistic regression, and p
A total of 307 participants were recruited to the study, comprising 187 patients
with IS and 120 healthy control volunteers. The demographic characteristics for
these two groups are summarized in Table 2. No significant differences in gender,
smoking habits, alcohol consumption, TG and HDL-C levels were found between the
IS and control groups (p
Characteristic | Controls (n = 120) | IS patients (n = 187) | p-value |
Male (n, %) | 64, 53.33% | 97, 51.87% | 0.80 |
Age (years, mean |
60.03 |
64.23 |
|
Smoking (n, %) | 38, 31.67% | 64, 34.22% | 0.64 |
Drinking (n, %) | 31, 25.83% | 45, 24.06% | 0.73 |
Hypertension (n, %) | 52, 43.33% | 109, 58.29% | 0.01 |
Diabetes mellitus (n, %) | 25, 20.83% | 65, 34.76% | 0.01 |
TG (mmol/L, mean |
1.72 |
1.80 |
0.58 |
TC (mmol/L, mean |
4.86 |
5.13 |
0.04 |
LDL-C (mmol/L, mean |
3.37 |
3.59 |
0.03 |
HDL-C (mmol/L, mean |
1.38 |
1.29 |
0.07 |
SD, Standard deviation; TG, Triglyceride; TC, Total cholesterol; HDL-C, High density lipoprotein cholesterol; LDL-C, Low density lipoprotein cholesterol; IS, Ischemic stroke.
The genotype distribution of the 4 GABRG2 SNP loci did not differ
significantly from HWE (p
SNP | Genotype | Controls (n, %) | p-value | IS patients (n, %) | p-value |
rs211037 | CC | 68 (56.67) | 0.90 | 134 (71.66) | 0.11 |
TT | 7 (5.83) | 8 (4.28) | |||
CT | 45 (37.50) | 45 (24.06) | |||
rs418210 | CC | 30 (25.00) | 0.26 | 56 (29.95) | 0.91 |
TT | 24 (20.00) | 39 (20.86) | |||
CT | 66 (55.00) | 92 (49.19) | |||
rs211035 | AA | 16 (13.33) | 0.53 | 28 (14.97) | 0.57 |
GG | 44 (36.67) | 75 (40.11) | |||
AG | 60 (50.00) | 84 (44.92) | |||
rs424740 | AA | 6 (5.00) | 0.49 | 9 (4.81) | 0.44 |
GG | 78 (65.00) | 122 (65.24) | |||
AG | 36 (30.00) | 56 (29.95) |
HWE, Hardy-Weinberg equilibrium; SNP, Single nucleotide polymorphism; IS, Ischemic stroke.
GABRG2 SNP allele and genotype frequencies were
compared between the control and IS groups (Table 4). The allele and genotype
frequencies for rs211037 were significantly different between the two
groups (p
Allele (n, %) | p-value | OR (95% CI) | Genotype (n, %) | p-value | ||||
rs211037 | C | T | CC | TT | CT | |||
Control | 181 (75.42) | 59 (24.58) | 0.01 | 1.673 [1.119 |
68 (56.67) | 7 (5.83) | 45 (37.50) | 0.03 |
IS | 313 (83.69) | 61 (16.31) | 134 (71.66) | 8 (4.28) | 45 (24.06) | |||
rs418210 | C | T | CC | TT | CT | |||
Control | 126 (52.50) | 114 (47.50) | 0.62 | 1.086 [0.784 |
30 (25.00) | 24 (20.00) | 66 (55.00) | 0.56 |
IS | 204 (54.55) | 170 (45.45) | 56 (29.95) | 39 (20.86) | 92 (49.19) | |||
rs211035 | A | G | AA | GG | AG | |||
Control | 92 (38.33) | 148 (61.67) | 0.82 | 0.963 [0.689 |
16 (13.33) | 44 (36.67) | 60 (50.00) | 0.68 |
IS | 140 (37.43) | 234 (62.57) | 28 (14.97) | 75 (40.11) | 84 (44.92) | |||
rs424740 | C | G | CC | CG | GG | |||
Control | 48 (20.00) | 192 (80.00) | 0.95 | 0.987 [0.657 |
6 (5.00) | 78 (65.00) | 36 (30.00) | 0.99 |
IS | 74 (19.79) | 300 (80.21) | 9 (4.81) | 122 (65.24) | 56 (29.95) |
OR, Odds ratio; CI, Confidence intervals; IS, Ischemic stroke.
Linkage disequilibrium analysis was conducted on the 4 GABRG2 SNPs. The
D’ values between the 4 SNPs were all
SNP | rs418210 | rs211035 | rs424740 |
rs211037 | 0.094 | 0.035 | 0.030 |
rs418210 | — | 0.020 | 0.066 |
rs211035 | — | — | 0.119 |
SNP, Single nucleotide polymorphism.
Haplotype | IS (freq) | Control (freq) | p-value | OR (95% CI) |
C C A A | 12.94 (0.035) | 12.92 (0.054) | 0.258894 | 0.637 [0.289 |
C C A G | 50.26 (0.134) | 23.10 (0.096) | 0.140473 | 1.480 [0.877 |
C C G A | 14.30 (0.038) | 3.78 (0.016) | 0.103146 | 2.514 [0.799 |
C C G G | 90.74 (0.243) | 50.64 (0.211) | 0.323549 | 1.218 [0.823 |
C T A A | 13.60 (0.036) | 10.09 (0.042) | 0.742100 | 0.870 [0.379 |
C T A G | 43.39 (0.116) | 19.24 (0.080) | 0.139160 | 1.527 [0.869 |
C T G A | 14.64 (0.039) | 11.85 (0.049) | 0.560834 | 0.793 [0.362 |
C T G G | 73.12 (0.196) | 49.37 (0.206) | 0.807565 | 0.951 [0.633 |
T C A G | 6.07 (0.016) | 13.57 (0.057) | 0.006102 | 0.278 [0.105 |
T C G A | 7.74 (0.021) | 6.52 (0.027) | — | — |
T C G G | 17.93 (0.048) | 15.47 (0.064) | 0.396196 | 0.739 [0.366 |
T T A G | 9.58 (0.026) | 13.08 (0.055) | 0.067925 | 0.461 [0.197 |
T T G A | 6.63 (0.018) | 2.83 (0.012) | — | — |
T T G G | 8.90 (0.024) | 7.52 (0.031) | 0.585925 | 0.761 [0.284 |
T C A A | 4.00 (0.011) | 0.00 (0.000) | — | — |
T T A A | 0.14 (0.000) | 0.00 (0.000) | — | — |
Global result | 0.041351 |
IS, Ischemic stroke; OR, Odds ratio; CI, Confidence Interval.
In view of the significant differences observed between the IS and control groups for some clinical characteristics (age, hypertension, diabetes, TC, LDL-C), multivariate logistic regression analysis was conducted to exclude confounding variables (Table 7). The dependent variable was acute IS, while the included independent variables were age, hypertension, diabetes, TC, LDL-C and the rs211037 genotype. The TT genotype of rs211037 was found to be an independent risk factor for IS (OR = 1.925, 95% CI, 1.122–3.303, p = 0.017). Age was also an independent risk factor for IS, with older age being associated with a higher risk for IS (OR = 1.047, 95% CI, 1.020–1.073, p = 0.001).
Factor | p-value | OR (95% CI) | |
Age | 0.001 | 1.047 [1.020 | |
Hypertension | 0.095 | 0.622 [0.356 | |
Diabetes mellitus | 0.076 | 0.556 [0.291 | |
TC | 0.566 | 1.073 [0.842 | |
LDL-C | 0.094 | 1.317 [0.954 | |
rs211037 | |||
CC | 0.044 | — | |
TT | 0.017 | 1.925 [1.122 | |
CT | 0.960 | 0.969 [0.288 |
TC, Total cholesterol; LDL-C, Low density lipoprotein cholesterol; OR, Odds ratio; CI, Confidence Intervals.
We next analyzed the relationship between GABRG2 rs211037 genotype and the patients’ NIHSS score. Higher HIHSS scores were observed in TT genotype patients (Table 8). According to the TOAST classification, the TT genotype was significantly more frequent in LAA than the SAO and CE groups.
Genotype | NIHSS | TOAST | |||
Minor (n = 47) | Moderate (n = 140) | LAA (n = 68) | SAO (n = 70) | CE (n = 49) | |
CC | 30, 63.8% | 104, 74.3% | 52, 76.5% | 50, 71.4% | 32, 65.3% |
TT | 0, 0.0% | 8, 5.7% | 6, 8.8% | 1, 1.4% | 1, 2.0% |
CT | 17, 36.2% | 28, 20.0% | 10, 14.7% | 19, 27.2% | 16, 32.7% |
p-value | 0.030 | 0.042 |
NIHSS, National Institute of Health Stroke Scale; TOAST, Trial of ORG 10172 in Acute Stroke Treatment; LAA, Large-artery atherosclerosis; SAO, Small artery occlusion; CE, Cardiogenic embolism.
This study was conducted to investigate possible associations between GABRG2 (rs211037, rs418210, rs211035, and rs424740) genotypes and the onset of IS. Univariate analysis showed significant differences in the distribution of the rs211037 genotype between the control and IS groups. Our results indicate that patient age and the TT genotype of rs211037 may be independent risk factors for susceptibility to IS. In addition, IS patients with the rs211037 TT genotype had a higher NIHSS score, with the cause of stroke in these patients being mostly atherosclerosis of the large arteries. These findings suggest potential factors for the identification of IS-susceptible individuals in the population, while also providing a theoretical basis for the etiology of stroke.
SNPs in GABRG2 have been associated with certain diseases. For example, the rs211037 polymorphism in the GABRG2 gene has been linked to an increased risk of idiopathic generalized epilepsy [16]. A meta-analysis demonstrated that GABRG2 rs211037 was associated with susceptibility to epilepsy [10]. A study of Indian epilepsy patients reported the TC genotype frequency of the rs418210 locus in GABRG2 was significantly different between patients and controls (p = 0.011) [22]. Yin et al. [20] reported that individuals with the GA haplotype for the rs211035 and rs211034 SNPs were less likely to be suicidal. Another study by the same group also found the SNP rs424740 was associated with major depression [24]. However, to our knowledge there have been no published reports on whether GABRG2 SNPs are genetic susceptibility factors for IS. The current study investigated 4 SNPs in GABRG2 based on previous work: rs211037, rs418210, rs211035, and rs424740. Univariate analysis revealed that only rs211037showed different allele and genotype frequencies between control and IS groups. Clinical risk factors for stroke were also recorded in this study. The incidence of hypertension, diabetes, TC and LDL-C were significantly different between IS patients and healthy controls. We performed multivariate analysis to exclude the impact of these clinical factors on the evaluation of rs211037 genotype with regard to IS. The TT genotype of rs211037 was found to be an independent risk factor for IS. These results suggest the GABRG2 rs211037 SNP is an IS-related risk factor. Another study reported similar results [16], whereas other studies have suggested that GABRG2 rs211037 SNP does not influence susceptibility to epilepsy in different populations [25]. The GABRG2 SNPs rs211035, rs424740, rs418210, rs211034, and rs424740 may not be susceptibility factors for epilepsy in Indian patients [22]. The diversity of findings in these studies may be due to factors such as ethnic differences, study design, sample size, and disease species.
The pathogenesis and risk of IS also associated with a variety of clinical factors or features in addition to genetic factors [26]. For example, Egan et al. [27] found that blood pressure and increased morbidity and mortality of stroke were associated with hypertension. A Mendelian randomization study also found that type 2 diabetes was a risk factor for IS in both large and small arteries [28]. This was also confirmed in the present study, where in addition to the TT genotype of rs211037, patient age was shown to be an independent risk factor for IS. Hence, clinical features may also assist in the treatment of stroke. For example, some investigators reported that thrombus permeability in patients was a potential neuroimaging marker for predicting the risk of distal embolism in mechanical thrombectomy [29]. Therefore, a combined analysis of rs211037 SNP and patient age may improve the accuracy of prediction for IS risk.
Subgroup analysis has been a core problem in the clinical classification of IS and other diseases. This type of analysis can reduce the influence of phenotypic heterogeneity on disease diagnosis. A multicenter review found that large vessel occlusion was more suitable for thrombectomy compared to other subgroups [30]. The change of gene polymorphism was also closely related to the region, race and age of persons with IS. The IL-6 rs1800795 polymorphism is significantly correlated with IS depending on ethnicity and geography [31]. Subgroup analysis showed that the negative effect of the circular (circ)-STAT3 rs2293152 GG genotype was more pronounced in female and older patients [32]. The NIHSS score is a standard classification method for IS patients. SNPs in Nitric Oxide Synthase 1 show a negative effect in IS patients in the codominant and dominant categories [33]. With the TOAST classification, some researchers reported the 1082 A/G polymorphism of IL-10 was significantly associated with the risk of IS for large vessel disease (LVD) and small vessel disease (SVD) patients [34]. Similar to these results, we also found that patients with the TT genotype of rs211037 had higher NIHSS scores. This was the predominant genotype in the LAA subgroup of IS patients. In previous studies on LAA and other IS subtypes, researchers generally believed that etiological differences between subtypes were directly related to clinical characteristics [35]. In the present study, the rs211037 SNP was also regarded as a risk factor for the LAA subtype.
There are several limitations to this study. First, the number of patients and healthy volunteers was small, and the findings need to be verified by study of a larger sample size. Second, the study participants were all of Chinese population, hence it is not possible to extrapolate the significance of the results to other populations. Further analysis of different populations may clarify the actual significance of this genetic polymorphism. Third, only four polymorphic loci in GABRG2 were studied, and future work should study investigate other polymorphisms in the GABRG2 gene for possible associations with stroke. Finally, this study was focused on clinical and genetic risk factors for IS and did not evaluate possible associations with prognosis. This may be a worthwhile future research direction.
In conclusion, univariate analysis showed the genotype distribution of the GABRG2 rs211037 SNP was significantly different between IS patients and healthy controls. Older patient age and the TT genotype of rs211037 may be independent risk factors for susceptibility to IS. In subgroup analysis, IS patients with the TT genotype of rs211037 were found to have higher NIHSS scores. The TT polymorphism of rs211037 was also the predominant genotype in the LAA subtype of IS. Our findings indicate the rs211037 SNP in GABRG2 may be a genetic susceptibility factor for IS in the Chinese population. The results of this study may help to transform genetic discoveries into disease prediction biomarkers. These results also provide useful information for understanding the complex causes of IS.
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
MM and JC conceived and designed the study, revised the draft. MM, JZ and DX conducted the experiments. JC wrote the first draft and revised the draft. MM and JZ led statistical analysis and revised the draft. JC led the revision of the draft. All authors contributed to editorial changes in the manuscript. All authors read and approved the final manuscript. All authors had complete access to all research data and assume complete responsibility for the data integrity and accuracy of the data analysis.
The experiments were approved by the Medical Ethics Committee of Red Cross Hospital. The ethical statement No. is 2023030. All subjects gave written informed consent in accordance with the Declaration of Helsinki.
Not applicable.
This work was supported by the Hangzhou Biomedicine and Health Industry Development Support. The project name is “Construction of Circulating Exosome S100B Detection System and Its Value in Stroke Diagnosis and Prognosis”. The Project Number is 2023WJC099.
The authors declare no conflict of interest.
Publisher’s Note: IMR Press stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.