- Academic Editor
†These authors contributed equally.
Introduction: Premonitory urge (PU) is an aversive bodily sensation
that signals the onset of tic disorder.To our knowledge, PU typically precedes
the appearance of tic symptoms, and both age and tic severity are correlated with
PU. However, inconsistent findings have also been reported. Hence, we conducted a
meta-analysis to examine the relationship among premonitory symptoms, patient age
and the severity of tic symptoms, as well as to summarize the research on the
neural underpinnings of PU in Tourette syndrome (TS). Methods: We
conducted a literature search of relevant studies published between December 2005
and April 2022 using databases such as PubMed, Elsevier, PsycINFO, and Web of
Science. Our analysis was carried out using R software with the assistance of the
“meta” and “metafor” packages. Results: Our meta-analysis included
22 studies with a total of 1236 tic disorder patients. The mean Premonitory Urge
for Tics Scale (PUTS) score was 20.17, with a 95% confidence interval of [18.14,
21.68]. Through meta-regression, we found that age and tic severity play
important moderating roles in PU severity (p
Tic disorder (TD) is a prevalent neurodevelopmental condition, with Tourette syndrome (TS) being its most severe form. Many patients with chronic TDs and TS report experiencing pressure-like or tickling sensations prior to the occurrence of tics [1, 2]. These sensations are well-known symptoms accompanying tics [3]. The term “premonitory urge (PU)” was first used by Leckman et al. [4]. The majority of patients describe these pressure, itch, and sore sensations as aversive or unpleasant before the onset of a tic. A prior study found that PU was present in nearly 90% of patients with TS [5]. The most common sites of PU are in the head and neck and are separate from the anatomic region where the tics occur [6].
PU plays a crucial role in the etiology of TD. Many studies have reported that patients can temporarily relieve the sensations associated with PU by producing tic symptoms. In other words, tics are often seen as a motor response aimed at reducing the intensity of PU [7]. For instance, the recognition of an itchy tongue as a PU could lead to tongue biting as a tic symptom, which in turn can alleviate the itchy sensation [1]. Our previous meta-analysis showed a mild to moderate correlation between PU strength and tic severity [8]. Additionally, PU has been shown to play a significant role in behavioral therapy for TS [9]. Awareness of PU before the onset of tics is the first step in habit reversal training, a widely used behavioral psychotherapeutic approach for individuals with TD. This approach highlights the importance of increased insight into PU in helping patients improve their ability to suppress tics. Pertinently, PU is a fundamental component in the formation of tics [10, 11]. Despite this, the mechanism underlying PU and its relationship with tic symptoms remains unclear.
The onset of PUs typically occurs around the age of 10 years and may be linked to the patient’s increasing awareness and cognitive development [4, 12, 13, 14]. However, some studies have reported an earlier onset of PU [15]. This may be due to a lack of introspection and the inability of younger children to describe feelings [15, 16]. The severity of PU is generally mild in children and adolescents, but it can become more pronounced in adults [7, 10, 17]. These findings suggest that age is an important factor influencing PU, although the relationship between patient age and PU severity still requires further investigation.
Moreover, the underlying neurobiological mechanism of PU remains unclear. However, several studies have implicated the anterior insula as a potential source of PU [18, 19]. One study using functional magnetic resonance imaging (fMRI) found that a region in the right insular cortex was associated with PU scores [19]. Other neuroimaging studies, including functional and structural magnetic resonance imaging (MRI), have suggested that PU may be related to activity in the prefrontal cortex (PFC), anterior cingulate cortex (ACC), and supplementary motor area (SMA) [13, 20, 21]. These findings suggest that the generation of PU may involve multiple brain networks. Further research is needed to explore the relationship between PU and tics, particularly from a network perspective.
The aim of this meta-analysis was to examine the predictors of PU, particularly patient age and tic symptom severity. We conducted a meta-regression analysis to achieve this goal. This study also examined the relationship between PU and tic symptom severity. Additionally, to gain a deeper understanding of PU, we focused on magnetic resonance imaging (MRI) studies and aimed to present an updated summary of the evidence-based neural correlates of PU.
This systematic review and meta-analysis was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) [22] and was registered with the International Prospective Register of Systematic Reviews (PROSPERO Register CRD42022379230). PRISMA checklist is shown in Supplementary material.
We conducted a comprehensive literature search for relevant studies published between December 2005 and April 2022 in PubMed, Elsevier, PsycINFO, and Web of Science. Our main search terms included “premonitory urge”, “sensory phenomena”, and various variants of “tic disorder” or “Tourette syndrome”. We also manually checked the reference lists of the included studies to ensure completeness.
To be eligible for inclusion in our meta-analysis, studies had to meet the following criteria: assessment of PU using the Premonitory Urge for Tics Scale (PUTS), assessment of tic severity using the Yale Global Tic Severity Scale (YGTSS), and inclusion of both child and adult subjects. Studies were excluded if they failed to meet any of the following criteria: no mention of PU score, lack of focus on TDs, not original research, and sample size less than 10.
Additionally, we performed a separate search using the terms “premonitory urge” and “MRI” or “imaging” to identify studies examining the neural correlates of PU.
The following data were extracted from each study: first author, publication date, sex and age of participants, sample size, mean value and standard deviation (SD) of PU as assessed by the PUTS, mean value and SD of tic severity as assessed by the YGTSS. In addition, the main findings from included MRI studies examining the neural correlates of PU were documented.
The quality of each study was evaluated using a modified version of the critical appraisal skills program (CASP) scale [23, 24]. This widely used tool assesses the strengths and limitations of qualitative research methodologies and includes 11 items, such as “Item 1: Did the study address a clearly defined issue?” and “Item 4: Were the controls chosen appropriately?” Two authors independently assessed each included trial and reached a consensus. Studies were excluded if they had fewer than 5 ‘yes’ responses on the CASP scale.
We employed the use of a pooled mean with 95% confidence intervals (CIs) to
quantify PUs and conducted a meta-regression analysis to examine the predictors
of PUs, specifically patient age and the severity of tic symptoms. To assess the
heterogeneity of each effect size, we utilized the I
This meta-analysis was conducted in accordance with the PRISMA guidelines and resulted in the inclusion of 22 studies [25] that covered a wide age range of participants between 6 and 50 years old (as shown in Fig. 1). A total of 1236 patients were included in these studies (as outlined in Table 1) [14, 17, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42], and the quality assessment of the included studies can be found in Supplementary Table 1 (which is also included in the supplementary material as Supplementary Fig. 1).
Flowchart of the included studies. PU, Premonitory urge.
Study | Year | Male (%) | Age (Year) | AgeG (1/2) | N | PU (Mean) | PU (SD) | YGTSS (Mean) | YGTSS (SD) |
Anis et al. 2022 [31] | 2022 | 61.1 | 30.5 | 2 | 18 | 22.9 | 5.5 | 25.3 | 17.1 |
Ramsey et al. 2021 [32] | 2021 | 60 | 10.68 | 1 | 75 | 16.46 | 6.03 | 23.53 | 8.39 |
Fu et al. 2021 [36] | 2021 | 20 | 19.7 | 2 | 15 | 21.13 | 6.749 | 30.13 | 8.601 |
Jackson et al. 2021 [39] | 2021 | 89.3 | 14.62 | 1 | 28 | 20.21 | 6.81 | 22.9 | 19.25 |
Bhikram et al. 2020 [34] | 2021 | 80 | 37.1 | 2 | 40 | 25 | 4.8 | 25.6 | 8.95 |
Baumung et al. A 2021 [33] | 2021 | 78.5 | 34.2 | 2 | 93 | 23.84 | 6.25 | 20.34 | 9.18 |
Baumung et al. B 2021 [33] | 2021 | 83.1 | 11.8 | 1 | 148 | 21.08 | 6.32 | 20.2 | 9.18 |
Matsuda et al. A 2020 [40] | 2020 | N/A | 28.5 | 2 | 25 | 22.9 | 6.9 | 23.4 | 7.8 |
Matsuda et al. B 2020 [40] | 2020 | N/A | 12.9 | 1 | 37 | 21.2 | 6.9 | 19.4 | 7.2 |
Yi Gu et al. 2020 [37] | 2020 | 81 | 9.5 | 1 | 252 | 12.96 | 3.23 | 19.63 | 9.2 |
Sigurdsson et al. A 2020 [41] | 2020 | 89.3 | 14.6 | 1 | 28 | 20.2 | 6.8 | 22.9 | 19.3 |
Isaacs et al. 2020 [38] | 2020 | 70.6 | 33.5 | 2 | 34 | 24.9 | 4.9 | 24.9 | 10.7 |
Sigurdsson et al. B 2020 [42] | 2020 | 68.8 | 20.4 | 2 | 16 | 22.94 | 6.4 | 21 | 14.5 |
Brandt et al. A 2016 [26] | 2016 | 86.4 | 29.82 | 2 | 22 | 21.05 | 5.78 | 17.05 | 7.7 |
Brandt et al. B 2016 [26] | 2016 | 77.5 | 12.05 | 1 | 40 | 17.83 | 6.38 | 17.77 | 8.12 |
McGuire et al. 2016 [30] | 2016 | 60 | 10.68 | 1 | 75 | 16.46 | 6.03 | 23.53 | 8.39 |
Rozenman et al. 2015 [28] | 2015 | 78 | 11.76 | 1 | 134 | 17.34 | 6.63 | 24.65 | 6.09 |
Kano et al. 2015 [29] | 2015 | 76 | 23.1 | 2 | 41 | 18.6 | 6 | 20.8 | 19.1 |
Capriotti et al. 2014 [35] | 2014 | 86.7 | 11.4 | 1 | 15 | 18.2 | 7 | 22.1 | 4.7 |
Sutherland Owens et al. 2011 [17] | 2011 | 72.2 | 19.35 | 2 | 18 | 25.8 | 5 | 24.2 | 8 |
Steinberg et al. 2010 [27] | 2010 | 85 | 11.05 | 1 | 40 | 20.15 | 5.89 | 18.15 | 7.76 |
Woods et al. 2005 [14] | 2005 | 92.9 | 10.8 | 1 | 42 | 18.5 | 6.1 | 25.9 | 10.1 |
Note. AgeG1, child group; AgeG2, adult group; PU, premonitory urge; YGTSS, Yale Global Tic Severity Scale; SD, standard deviation.
The results of our meta-analysis showed that the average PUTS score was 20.17,
with a 95% CI of [18.76, 21.69] and I
The meta-analysis for the mean PU. 95% CI, 95% confidence interval; SD, standard deviation.
The source of heterogeneity was explored through subgroup analysis, which showed
a significant difference between adult and child participants. The mean PUTS
score in the adult group was 22.98 (95% CI [21.58, 24.47], I
Subgroup analysis for the mean PU.
The results showed that age was a significant predictor of PU, with tau
Meta-regression analysis for PUs by age and tic severity. PU, Premonitory urge; PUTS, Premonitory Urge for Tics Scale.
Both MRI and magnetic resonance spectroscopy (MRS) were employed to investigate the neural basis of PU at both functional and structural levels. Our analysis identified 12 relevant studies (refer to Fig. 2). In terms of functional aspects, the results showed that PU severity as measured by PUTS scores and the inhibition of PU were correlated with decreased connectivity between the cortex and insula seeds, as revealed by resting-state functional MRI [19, 34]. On the other hand, functional connectivity between the right dorsal anterior insula and bilateral SMA was found to positively correlate with PU severity [18]. At the structural level, structural MRI showed an inverse association between PU and gray matter volume in the insula and sensorimotor cortex [13]. Additionally, increased activity in other regions, such as the ACC and right temporoparietal junction, was observed during the inhibition of PU [43, 44]. Furthermore, gray matter volume in the cerebellar lobule was implicated in sensorimotor processing, which is related to PU [41, 45]. Finally, Gamma-aminobutyric acid (GABAergic) processing was found to be crucial in sensorimotor processing [46]. Gamma-aminobutyric acid (GABA)-edited MRS studies revealed lower levels of GABA in the primary sensorimotor cortex (SM1) in children with TS and a correlation between more severe and frequent PUs with lower levels of SMA GABA+ [47]. A summary of these findings can be found in Table 2 (Ref. [13, 18, 21, 34, 43, 48, 49, 50, 51, 52, 53, 54, 55]).
Study | Sex (M/F) | Age | Imaging method | Measurement | Main findings |
Cavanna et al. (2008) [48] | TS: 30/10 | TS: 32.4 |
VBM analysis | YGTSS; PUTS | Premonitory urges had an impact on the integrity of tracts corresponding to cortico-cortical and cortico-subcortical connections. |
HC: 25/15 | HC: 34.4 | ||||
Hampson et al. (2009) [49] | - | TS: 30.0 |
Coactivating analysis | - | The SMA showed a significantly broader profile of cross-correlation with the motor cortex during tics than during intentional movements. |
HC: 30.0 | |||||
Wang et al. (2011) [50] | TS: 8/5 | TS: 33.5 |
Activity and connectivity analysis | YGTSS; Y-BOCS; ADHS | Tics are caused by the combined effects of excessive activity in motor pathways and reduced activation in control portions of cortico-striato-thalamo-cortical circuits. |
HC: 12/9 | HC: 32.5 | ||||
Ganos et al. (2014) [51] | TS: 13/1 | TS: 30.6 |
ReHo analysis | YGTSS; MRVS; PUTS; Y-BOCS; ADHS; BDI | ReHo of the left inferior frontal gyrus was increased during voluntary tic inhibition compared to that during free ticcing. |
Tinaz et al. (2015) [18] | TS: 10/3 | TS: 18–46 years | Theory-based neural network analysis | YGTSS; PUTS | The right dorsal anterior insula is part of the urge-tic network and could influence the urge- and tic-related cortico-striato-thalamic regions even during rest in TS. |
HC: 10/3 | HC: 22–56 years | ||||
Draper et al. (2016) [13] | TS: 26/3 | TS: 14 |
Gray matter thickness analysis | YGTSS; PUTS; WASI | Premonitory sensory phenomena are inversely associated with gray matter thickness measurements within the insula and sensorimotor cortex. |
HC: 26/3 | HC: 14 | ||||
Eddy et al. (2016) [43] | TS: 19/6 | TS: 31.48 |
Task-fMRI (theory of mind) | YGTSS; PUTS; Y-BOCS; ASRS; HADS; MIDI | Activity within the right temporo-parietal junction and amygdala as localized by this task covaried with PUs. |
HC: 19/6 | HC: 29.88 | ||||
Kanaan et al. (2017) [52] | TS: 31/6 | TS: 38.3 |
MRS | YGTSS; RVTRS; PUTS; Y-BOCS; OCI-R; BDI-II | Thalamic Glu and PUs had a significant negative correlation. |
HC: 29/7 | HC: 38.4 | ||||
Sigurdsson et al. (2018) [53] | TS: 32/3 | TS: 14.0 |
DTI tract-based spatial statistics and probabilistic tractography | YGTSS; PUTS; CY-BOCS; Conners-3 self-report; SCQ; WASI | The frequency of PU was associated with increased connectivity between M1 and the caudate nucleus, and increased information transfer between M1 and the insula, respectively. |
HC: 32/3 | HC: 13.9 | ||||
Botteron et al. (2019) [54] | TS: 6/9 | TS: 28.0 |
Task-fMRI (urge to blink) | YGTSS; DCI; PUTS; Y-BOCS; ASRS | Combining this approach with observed eye closure during fMRI blink suppression trials should extract brain signals more tightly linked to the urge to blink. |
HC: 6/9 | HC: 26.0 | ||||
Rae et al. (2020) [21] | TS: 13/8 | TS: 33.0 |
Task-fMRI (go/no-go task) | YGTSS; PUTS; Y-BOCS; ASRS | SMA connectivity varied in proportion to premonitory sensations. |
HC: 11/10 | HC: 34.0 | ||||
Bhikram et al. (2020) [34] | TS: 32/8 | TS: 37.1 |
Seed-based FC analysis | YGTSS; Y-BOCS; PUTS; ASRS | PU severity was associated with less connectivity between the orbitofrontal cortex and sensorimotor cortex and among the inferior frontal gyrus, the putamen and insula seeds. |
HC: 16/4 | HC: 36.9 | ||||
Bhikram et al. (2021) [55] | TS: 31/8 | TS: 37.1 |
Task-fMRI (blink inhibition and free blinking) | YGTSS; Y-BOCS; WBSI; USP-SPS; PUTS | Greater premonitory urge severity was associated with greater activity in the hippocampus, middle temporal gyrus and subcortex. |
HC: 16/4 | HC: 36.9 |
Note: TS, Tourette syndrome; HC, healthy control; N, number; M, male; F, female; VBM, voxel-based morphometry; ReHo, regional homogeneity; fMRI, functional magnetic resonance imaging; MRS, magnetic resonance spectrum; DTI, diffusion tensor imaging; FC, functional connectivity; YGTSS, Yale Global Tic Severity Scale; PUTS, Premonitory Urge for Tics Scale; Y-BOCS, Yale-Brown Obsessive-Compulsive Scale; ADHS, ADHD self-assessment scale; MRVS, Modified Rush Video Scale; BDI, Beck Depression Inventory; WASI, Wechsler’s Abbreviated Scale of Intelligence; ASRS, ADHD Self-Report Scale; HADS, Hospital Anxiety and Depression Scale; MIDI, Minnesota Impulsive Disorders Interview; RVTRS, Rush Video-based Tic Rating Scale; OCI-R, Revised Obsessive-Compulsive Inventory; SCQ, Social Communication Questionnaire; DCI, Diagnostic Confidence Index; WBSI, White Bear Suppression Inventory; USP-SPS, University of São Paulo Sensory; SMA, supplementary motor area; M1, motor cortex; CY-BOCS, Children’s Yale-Brown Obsessive- Compulsive Scale.
To our knowledge, this was the first study to conduct a meta-analysis investigating the relationship between PU and patient age. Our findings indicated that younger individuals tended to have a lower level of PU, while older individuals tended to experience a higher level. This could be because with age, children develop greater self-awareness and the ability to describe their sensory experiences [12, 28]. As a result, the reporting of PU increases [4, 12]. By age 10, children are better able to describe the sensations they experience and report more awareness of PU [4, 5, 56]. On the other hand, while the tic symptoms of some patients start to subside at approximately 17 years of age, approximately 23% of severe tic cases become intractable [57, 58, 59]. Additionally, the PUTS tool for PU assessment is self-reported, and its diagnostic validity in young patients is limited, making it challenging to use in this population [26]. Therefore, developing a structured interview to assess PU in young patients may be a valuable direction for future research. Traditionally, PUs have often been observed to present three years after the onset of tics [60]. In addition, a recent study found that associations between reported urge levels and instantaneous tic intensity tended to be less pronounced in children and adolescents than in adult TS patients, which also supports the age effect in PU [61].
Furthermore, tics can be voluntarily suppressed for a brief period [62]. Thus, PU is a valuable cue for tic suppression. Behavioral treatments, such as habit reversal therapy (HRT), are considered a promising therapy for tics [8, 63]. HRT, which is widely used to treat TS, has been confirmed by numerous studies to be effective [64, 65, 66]. For instance, a meta-analysis of HRT showed that PU is closely related to tic symptoms [8, 67]. Detecting PU is the first step in HRT [68]. The therapy aims to increase awareness of urges and to help patients develop coping behaviors to replace tic symptoms [69]. This implies that greater awareness of PU leads to improved HRT efficacy. Furthermore, several studies have reported that PUs may play a crucial role in maintaining TD and have confirmed that PUs have a strong association with tic symptoms [11, 60].
The widely accepted neurobiological basis for TS is dysfunction of the cortico-striatal-thalamo-cortical (CSTC) circuit, as supported by numerous neuroimaging studies [13, 39, 45, 70]. These studies have shown that aberrant activity in the striatum may be linked to PUs. Structural MRI data, such as gray matter volume, cortical thickness, and connectivity matrices obtained from resting/task-state fMRI data, provide valuable morphological insights into PUs. Decreased gray matter thickness in the insula and sensorimotor cortex has been linked to PUs in young people with TS [13]. Whole-brain analysis of cortical (gray matter) thickness demonstrated that in young adults with TS, a reduction in cortical thickness within the insula, sensorimotor cortex, anterior cingulate, and insula was associated with higher ratings of PU severity and was significantly lower than that in a group of typically developing controls [13].
At the neurochemical level, dopaminergic modulation of glutamatergic corticostriatal afferents has been linked to the expression of diverse motor and nonmotor symptoms, including those seen in TS [71]. This anatomical substrate is reflected in the lower functional connectivity between the orbitofrontal cortex and sensorimotor cortex, between the inferior frontal gyrus and the putamen, and between the inferior frontal gyrus and insula as PU severity increases [34, 43]. The SMA shows a broader profile of cross-correlation with the motor cortex during tics compared to that during intentional movements, with its proportion varying with premonitory sensations [18]. Increased PU frequency was found to be associated with greater connectivity between the primary motor cortex (M1) and the caudate nuclei and increased transfer of information between M1 and the insula [72]. Moreover, greater PU severity was associated with greater activity in the hippocampus, middle temporal gyrus and subcortex [55].
The insula has been indicated to play a critical role in PU, suggesting that it is associated with the urge-to-tic [19]. The brain network involving the insula, ACC, and SMA is associated not only with the neural mechanism of PU but also with tic symptoms [73, 74]. Further imaging studies are needed to better understand the relationship between PU and tic symptoms in the future.
Overall, the current neuroimaging research suggests that PUs may be an early component of a network that includes the insula, ACC, and SMA. The insula appears to play a key role in the generation of PUs, connecting the sensory and emotional aspects of PUs. Moreover, the ACC and SMA may be involved in the urge-to-tic. These areas are recognized as important nodes of the salience network, suggesting that this network may play a role in both urge generation and the urge-to-tic. Further studies are required to fully understand PUs at the network level.
In addition to the generation of PUs, PU inhibition is also an important aspect to consider. Evidence from multiple studies has indicated that TS patients exhibit dysfunction in the inhibition of PUs [75, 76, 77]. This evidence highlights the need to investigate the role of inhibition in the development of PUs. Of note, impaired automatic inhibition has been reported [78]. We speculate that the generation and inhibition of PUs appear to be interrelated processes, and an imbalance between them may contribute to an increased urge-to-tic. Whether PUs translate into tics may depend on the balance between these two processes.
To advance PU research, a reliable and validated measure of sensory symptom severity is crucial. One widely used instrument is the PUTS, which was developed by Woods and his team [14]. The PUTS was optimized to nine items and has been widely used in clinical practice [78, 79, 80]. Multiple studies have provided evidence for the reliability and validity of PUTS [21, 26, 81]. However, new assessment tools have also been developed, including the Individualized Premonitory Urge for Tics Scale (I-PUTS) [30] and the Rumination and Awareness Scale for Tic-associated Sensations (RASTS) [40]. While these tools provide new insights into PUs, they also have limitations, and further development is necessary. The inclusion of multiple dimensions, such as location, severity, frequency, and intensity, is important for future advancements in PU assessment.
This meta-analysis supports the correlation between PUs and tic symptoms, reinforcing the significance of PUs in the treatment of TDs. The findings indicated that patient age is a crucial moderator affecting PU severity, implying that the impact of age should be considered during PU assessments. Considering these results, future research should be directed toward further understanding the nature of PUs. However, several challenges need to be addressed in future studies. First, current PU assessment tools are subjective and lack objectivity. New tools such as the I-PUTS and the RASTS require further validation across different countries and languages. Second, neuroimaging studies face difficulties in separating the processing of PUs and tic symptoms. Thus, future studies should aim to explore PUs at the neural circuitry level. Third, while computational psychiatry approach holds potential in understanding PUs, it requires further development to align with clinical practices. Finally, considering dopaminergic hyperinnervation in TS, investigating PUs based on this aspect may also be worthwhile.
There were two limitations to this study. First, the sample size of the included studies was small, which may have affected the accuracy of the results. Second, most of the included studies were from clinical samples with different inclusion and exclusion criteria, which might increase the heterogeneity of the meta-analysis.
The present meta-regression study confirmed the positive correlation between the severity of tics and PUs. Moreover, patient age appears to be a significant influencing factor for PU, as evidenced by the increasing PUTS score with age and tic severity. Neural correlates of PUs were associated with the insula, ACC and SMA, as well as the network associated with these regions. To further support these findings, future studies with larger sample sizes are necessary.
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
YLL and LPY were responsible for the extraction and drafting of the manuscript, as well as the analysis of data. HZ and XBW assisted with the search for relevant articles and creation of figures. YL and YHC were involved in the design and revision of the manuscript. All authors contributed to editorial changes in the manuscript. All authors have read and approved the final manuscript, and have contributed significantly to the work. All authors have agreed to take responsibility for all aspects of the study.
Not applicable.
Not applicable.
This work was supported by the National Natural Science Foundation of China (NSFC) under Grant No. 82001445, No. 82171538 and the Natural Science Foundation of Beijing Municipality under Grant No. 7212035, Beijing Hospitals Authority Youth Programme Grant No. QML20211203.
The authors declare no conflict of interest.
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