Fig. 1.Establishment of murine enteric neuron primary culture system.
(A) Summary of experimental procedures (Created with BioRender.com). (B)
Representative images of primary cultured cells derived from longitudinal
muscle/myenteric plexus (LMMP) obtained from the small intestine of 8-week-old
C57BL/6J mice (male), fixed at 8 DIV. The cells were immunostained with
antibodies specific for III-Tubulin (green), a marker of neurons; GFAP
(red), a marker of glia; and Hoechst (blue) for cell nuclei staining (Scale bar:
50 µm). DIV, days in vitro. GFAP, glial fibrillary acidic
protein.