Background: Immune escape is a key factor influencing survival rate of
lung adenocarcinoma (LUAD) patients, but molecular mechanism of ubiquitin binding
enzyme E2T (UBE2T) affecting immune escape of LUAD remains unclear. The objective
was to probe role of UBE2T in LUAD. Methods: Bioinformatics means were
adopted for analyzing UBE2T and forkhead box A1 (FOXA1) expression in LUAD
tissues, the gene binding sites, the pathway UBE2T regulates, and the correlation
between UBE2T and glycolysis genes. Dual luciferase and chromatin
immunoprecipitation (ChIP) assays were conducted for validating the binding
relationship between the two genes. Quantitative reverse transcription polymerase
chain reaction (qRT-PCR) and western blot were employed to evaluate UBE2T, FOXA1,
and programmed death ligand 1 (PD-L1) levels in cancer cells. MTT assay was conducted for detecting cell
viability. Cytotoxicity assay detected CD8
Announcements
Open Access
Original Research
FOXA1/UBE2T Inhibits CD8+T Cell Activity by Inducing Mediates Glycolysis in Lung Adenocarcinoma
Jiangtao Pu1,*, Dengguo Zhang1, Biao Wang1, Peiquan Zhu1, Wenxing Yang1, Kaiqiang Wang1, Ze Yang1, Qi Song1
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1
Department of Thoracic Surgery, Affiliated Hospital of Southwest Medical University, 646000 Luzhou, Sichuan, China
*Correspondence: drjiangtaop@163.com (Jiangtao Pu)
Front. Biosci. (Landmark Ed) 2024, 29(4), 134;
https://doi.org/10.31083/j.fbl2904134
Submitted: 25 August 2023 | Revised: 23 November 2023 | Accepted: 5 January 2024 | Published: 1 April 2024
Copyright: © 2024 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract
Keywords
FOXA1
UBE2T
glycolysis
lung adenocarcinoma
CD8+T cells
Figures
Fig. 1.